BS EN 15110:2006 pdf download
BS EN 15110:2006 pdf download.Water quality一 Guidance standard for the sampling of zoop an kton from standing waters.
When shallow lakes or littoral areas with a great deal of vegetation are being quantitatively sampled the use of a volume sampler, plankton pump or flexible tube is recommended
5.2.2 Other field equipment
The equipment listed in 5 1 2 (excluding the lead weight) and in addition a mixing vessel (eg. plastic bucket or similar) to combine a number of individual samples into a single sample in the held. Conducting mixed samples may be necessary to reducing analysis times and costs
If a volume sampler is being used )wTltl the exception of a Schindler-Patalas trap) filtration equipment is also required to concentrate the samples. This may take the form of either a plankton net (mesh size 45 pm, or 90 pm if only austacean plankton are being collected) or a large tunnel with draining cup fitted with a netting
5.3 Storage
Bottles (100 rr. 200 ml or 250 ml brown bottles with screw-tops) or glass vials for stonng samples.
Labels or tape to attach to the outside of the sample bottles Waterproof paper for labels to put inside the sample bottles
Marler pen If ethanol is being used. an alcotiol•prool pen or pencil is recorTirnended for both internal and external marking.
NOTE P’astic vials are not suitable foi storing samples if Lugol’s Iodine is used as preserving solution
6 Preserving solutions
6.1 General
A number of different preserving solutions with different areas of application are available The advantages and disadvantages of each of these solutions are defined in Annex A.
6.1.1 Formaldehyde 37 % by volume
This is netitralised, e.g with hexamethyl tetramine (CH..N4) Dilute the formaldehyde with water to 20 % (vtv) to avoid precipitation, and men add 1009 of hexamemyl tetramine and 409 to 80 g sucrose ((6]) per litre of 20 % formaldehyde.
WARNING — Formaldehyde may trigger allergies or cancers, and should therefore be handled with care.
6.1.2 Ethanol 96 % or 99 % C2H5OH
6.1.3 Lugols londine
Acidified Lugol’s Iodine Dissolve 1009 KI (potassium iodide) in ii of distilled or demineralised water, then add 50 g iodine (crystalline), shake until it is dissolved and add 100 ml of glacial acetic acid As this solution is close to saturation any precipitate should be removed by decanting the solution before use
Most preservatives are also commercially available For more details on the use of different preservatives the reader is referred to Annex A
Preserving solutions for field use should be kept in small stoppered bottles and should be accompanied by a pipette for transferring the solution to the plankton samples The bottles should be kept in a plastic box or container with lid during transportation.
7 Preliminary stages
7.1 Documentation of strategies and methods
The fdlowing docuir.mion should be a atie ieore the start of field work:
description of objectives and strategy,
— description Of methods.
safety instructions;
personnel plan:
overview of equipment and instruments:
— registration forms:
— procedures for the maintenance of records and samples.
— quality assurance requirements according to prEN 14996.
7.2 Preparation of sampling equipment
After each day of sampling, the net should be washed in warm freshwater with detergent or in an ultrasonic water-bath in order to reduce clogging and ensure opbmurn filtration capacity.
Check that the netting in the plankton net and draining cups is completely free of holes and tears
Check that the line is securely attached to the plankton net/volume sampler.
Check that the plankton samplers closing mechanism is functioning well and that any seals are in order
It may be advantageous to label tne sample bottles and to add the requisite amounts of preserving solution to them before the start of fieldwork.
In order to prevent spreading of flora and fauna between water bodies, the sampling equipment should be disinfecled between uses in the different waters.
