BS EN 01186-11:2002 pdf download
BS EN 01186-11:2002 pdf download.Materials and articles in contact with foodstuffs – Plastics – Part 11: Test methods for overall migration into mixtures of C-labelled synthetic triglycerides.
Take the tubes from the oven or incubator and immediately remove the test specimens from the tubes. For those specimens which have t I4C.IabelIed synthetic triglycerides, allow the triglycerides to drain. Remove any adhering “C-labelled synthetic triglycerides by gently pressing between filter
‘4C-labelled
papers (3.3.12). Repeat the pressing procedure until the filter paper shows rio spots of synthetic triglycerides. For test specimens on supports, remove the Individual test pieces from the supports to carry out this operation. Clean the supports of triglycerides by washing with the extraction solvent arid replace the test pieces on them.
NOTE 7 lIthe procedixe in annex C is followed, retain the tubes containing the triglycerides. The tubes should be capped to prevent further change ii the moisture content of the triycefldes and the Karl Fischer determination of water content should be carried out as soon as possible
3.5.4 Final weighing of test specimens
3.5.4.1 For those specimens which did not require conditioning to obtain their initial masses (see
‘C-labeIled synthetic triglycerides
3.5.2.2). weigh all six test specimens, ie. the four that have been in
and the two that were in the empty tubes and record the mass of each test specimen.
3.5.4.2 If conditioning of the test specimens was carried out using the procedure in annex A then repeat the procedure.
3.5.4.3 If conditioning was carried out before the initial weighing using the procedure described wi annex 8 then carry out the procedure described in 8.4.
3.5.4.4 If it was decided that the procedure described in arwiex C was applicable to the test sample, than carry out that procedure.
3.5.4.5 If the final mass of each of the test specimens which have been Ni empty tubes is less than their initial mass by more than 2,0 mg, then volatile substances have been lost and adjustment may be made, see 10.4 of EN 1186-1:2002, to the final mass for each test specimen such that the values obtained are a measure of the migration of non-volatile substances only.
3.5.5 Extraction of absorbed ‘4C-labetled synthetic triglycerides
‘IC-labelled synthetic triglycerides,
NOTE Some types of plastics are known to retain some of the absorbed
In these cases extraction of C.-Iabelled synthetic triglycerides is inconiplete and a second extraction with a more polar solvent is rjired. see also 10.2 of EN 1188-1:2002.
Take tour flasks. 250 ml or 500 ml as appropriate to the size of the soxhiet type extractor (3.3.14) to be used for the extraction, and add sufficient extraction solvent (32.2) to allow cycling of the soxhiet type extractor (approxImately 200 ml or 400 ml, according to the size of the flask) with anti-bumping beads (3.3.13) to control boiling.
‘IC-labelled synthetic triglycerides into
Place the tour test specimens wt,id, have been in contact with
tour soxhlet type extractors, Couple each soxhlet to a flask containing the extraction solvent. Using cycles per hour, ensunng that the test pieces are totally submerged in the solvent during each soxhlet cycle, and that they remain separated from each other.
Drain all of the solvent from the soxhiat type extractors, remove the flasks from the soxhlel type extractors and evaporate the solvent almost to dryness using a rotary evaporator, or simple distillation
4C-labelIed synthetic apparatus (3.3.16), Transfer the remaining solutions containing the extracted
triglycerides to separate liquid scintillation vials, and wash each flask with three portions of liquid scintillation cocktail. Add the three washings to the respective individual vials.
Repeat the extraction of the test specimens for an additional 7 Ii, with diethyl ether (3.2.4).
If previous testing has established that all of the “C-labeTled synthetic triglycerides will be extracted from the test specimens during the first 7 h extraction then the second 7 h extraction may be omitted.
